Berger, Horney, and Libby published a way of extracting the natural carbon from bone. Many laboratories adopted this process which produced a gelatin assumed to consist primarily of collagen. This process is called “insoluble collagen extraction” in this database. Longin indicated that collagen might be removed in a form that is soluble allowed a larger level of decontamination regarding the test. Haynes introduced an approach of extracting the inorganic carbon from bone.
This technique had been considered ideal for used in places where collagen is seldom or defectively preserved in bones. Subsequent research cast question regarding the dependability with this technique.
Hassan yet others ; Hassan and Ortner, showed that the inorganic carbon included in bone tissue apatite is extremely at risk of contamination by either more youthful or older carbon within the burial environment. It now appears that insoluble collagen extractions frequently err regarding the young part, if after all Rutherford and Wittenberg, , whereas bone apatite can create many years either older or more youthful as compared to real age, frequently with a margin that is considerable.